Inhibition of DNA synthesis by hydroxyurea: structure-activity relationships.
نویسندگان
چکیده
The following comix>unds inhibited incorporation of thymidine-3H into the DNA of HeLa cells without significantly impairing cellular incorjwration of uridine-3H into RNA or loucine-3H into protein; the}' are listed in order of potency. Dihydro.xyurea > jV-methylhydroxyurea > .V-acetylhydroxyurea = hydroxyurea = A'-hydroxyguanidine = N-hydroxyurethane = AT-ethylhydroxyurea > 3-phenyl-l-hydroxyurea = formamidoxime > Ar-mcthylacetohydroxamic acid = Ar-methyl-
منابع مشابه
Effect of Hydroxyurea on Cellular Iron Metabolism in Human Leukemic CCRF-CEM Cells: Changes in Iron Uptake and the Regulation of Transferrin Receptor and Ferritin Gene Expression following Inhibition of DNA Synthesis1
Hydroxyurea inhibits cellular proliferation through action on ribonucleotide reducÃ-ase,an iron-dependent enzyme responsible for the synthesis of deoxyribonucleotides. Whereas previous investigations have examined the interaction of hydroxyurea with this enzyme, the action of hydroxyurea on other aspects of iron metabolism has not been studied in detail. In our study, incubation of CCRF-CEM cel...
متن کاملDifferential regulation of DNA synthesis by nitric oxide and hydroxyurea in vascular smooth muscle cells.
We investigated the influence of nitrovasodilators on DNA synthesis in cultured human aortic smooth muscle cells and explored the hypothesis that nitric oxide (NO) is directly involved in mediating the inhibitory effects of hydroxyurea on DNA synthesis. Both NO and hydroxyurea inhibited ongoing DNA synthesis and S phase progression in our cells. Exogenous deoxynucleosides partially reversed thi...
متن کاملNuclear ultrastructure of the transforming lymphocyte during inhibition of deoxyribonucleic acid synthesis with hydroxyurea.
The pattern of decondensation of heterochromatin in the transforming lymphocyte was found to be unaffected by hydroxyurea although DNA synthesis, which normally accompanies the latter part of this decondensation, was greatly inhibited. It is suggested that metabolic inhibition of DNA synthesis may result in atypical sites of DNA synthesis when the inhibitor is removed, since such sites are norm...
متن کاملRegulation of thymidylate synthetase in mouse leukemia cells (L1210).
L1210 mouse leukemia cells were synchronized by isoleucine deprivation followed by hydroxyurea treatment. Changes of thymidylate synthetase activity both in cell extracts and in the intact cells (in situ) were followed throughout the cell cycle. The enzyme activity assayed in situ, by measuring tritium released from [5-3H]deoxyuridine, changed markedly in relation to the DNA synthesis pattern, ...
متن کاملEffect of hydroxyurea on replication of bacteriophage T4 in Escherichia coli.
Hydroxyurea inhibited the replication of bacteriophage T4 in Escherichia coli B. The concentration of hydroxyurea required to inhibit net deoxyribonucleic acid (DNA) synthesis 50% was about 50-fold less than that required in uninfected cells. Even in the presence of high hydroxyurea concentrations, phage DNA was readily synthesized from the products of breakdown of the E. coli DNA, and viable p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Cancer research
دوره 27 3 شماره
صفحات -
تاریخ انتشار 1967